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KMID : 0603820080140030199
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Journal of Experimental & Biomedical Science
2008 Volume.14 No. 3 p.199 ~ p.201
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Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 Induces the Chemotactic Activity of Human Eosinophils
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Lee Ji-Sook
Kim In-Sik
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Abstract
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Eosinophil is an improtant leukocyte in the development of various inflammatory diseases. Monocyte chemoattractant protein-1 (MCP-1) acts as a key regulator on monocyte movement, and activation of T cells and NK cells. However, the role of MCP-1 in eosinophils remains to be solved. In the present study, we examined the effect of MCP-1 on eosinophil migration, using human eosinophilic EoL-1 cells as an in vitro model of eosinophils. The surface expression of CCR2 in EoL-1 cells was little detected but MCP-1 strongly induced EoL-1 cell migration in a dose-dependent manner. Increased chemotactic activity due to MCP-1 was blocked by pertussis toxin, a Gi/Go protein inhibitor and U73122, a phospholipase C (PLC) inhibitor. These results suggest that MCP-1 activates Gi/Go protein and PLC and this signal pathway is involved in eosinphil movement. This finding supports the elucidation of pathogenic mechanism of eosinophilic inflammation such as asthma and atopic dermatitis.
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KEYWORD
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Eosinophilis, MCP-1, Gi/Go protein, Phospholipase C
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